NEW KIT from New England Biolabs!
NEBNext® Low-bias Small RNA Library Prep Kit
The NEBNext Low-bias Small RNA Library Prep Kit is designed to generate libraries that accurately reflect the relative proportions of RNA species present in a sample. The kit has a straightforward gel-free protocol with a wide input range (1,000 ng – 0.5 ng Total RNA; 5 ng – 0.05 ng Enriched small RNA) and a fast, one-day workflow. The kit is multiplexable for up to 480 LV Unique Dual Index primer pairs. Primer pairs are available separately.
The kit captures small RNAs (<120 nt) with a 5´ phosphate and 3´ hydroxyl group, and can be used to identify microRNAs (miRNAs), transfer RNAs (tRNAs) and tRNA-derived fragments, small nucleolar RNAs (snoRNAs), piwi-interacting RNAs (piRNAs), and plant miRNAs.
Available in two sizes:
NEBNext® Low-bias Small RNA Library Prep Kit, 24 rxns (E3420S)
NEBNext® Low-bias Small RNA Library Prep Kit, 96 rxns (E3420L)
NEBNext Low-bias Small RNA Library Prep workflow
Library construction with the NEBNext Low-bias Small RNA Library Prep Kit is robust and streamlined and can be completed in less than 4 hours. This enhanced workflow features protocol optimizations that enable shorter reaction times with two bead-purification steps that enable increased throughput and ease of use.
Kit Advantages
- Analyzes all RNA species present without library prep-generated bias
- Simplifies and streamlines your library prep workflow (~3.5 hours), saving time
- Enables new insights from biologically relevant input amounts
- 18-month shelf life
- A single protocol for standard and 2´-O-methylated samples
- Expands insights with up to 480 compatible UDI primer pairs, available separately
Webinar
Watch NEB´s Webinar about the Low-Bias Small RNA Library Prep Kit and learn more. Register below to get a link to the Webinar.
Webinar - Breaking Through Bias to Redefine Precision in Small RNA Library Prep
