Solutions for Fluorescence Imaging of Live Cells, Fixed Cells and Tissue Samples

Science Hub

Live-cell imaging and cellular structures

Biotium CellBrite™ Steady

For long-term plasma membrane imaging in live cells. Ideal for monitoring cell morphology, migration and co-culture experiments.

Biotium MitoView™

For live-cell mitochondrial imaging. MitoView™ 633 is especially useful for monitoring mitochondrial membrane potential.

Biotium LysoView™

For fluorescence imaging of lysosome localization and morphology in live cells, with options across multiple fluorescence channels.

Biotium ViaFluor® Live Cell Microtubule Stains

For rapid and sensitive imaging of the microtubule cytoskeleton in live cells.

Vector VectaCell™ Trolox

An antifade additive for live-cell imaging that reduces phototoxicity and photobleaching during long imaging experiments.

 

Cell viability, cell death and apoptosis

Biotium Calcein AM & Viability/Cytotoxicity Assay Kit

For live/dead cell discrimination by fluorescence microscopy, plate imaging or flow cytometry. Calcein AM stains viable cells green, while Ethidium Homodimer III stains dead cells red.

Biotium Live-or-Dye™ Fixable Viability Stains

For live/dead discrimination before fixation and permeabilization. A strong choice when downstream antibody staining is required.

Biotium NucView® Caspase-3 Substrates

For real-time monitoring of apoptosis in live cells through caspase-3/7 activity.

Cayman JC-1 Mitochondrial Membrane Potential Assay Kit

For measuring mitochondrial membrane potential as an indicator of cell health and cellular stress.

Cayman ROS Detection Cell-Based Assay Kit

For direct measurement of general reactive oxygen species in living cells.

Fixed cells and tissues - immunofluorescence

Biotium CellBrite™ Fix & MemBrite™ Fix

For membrane labeling in live cells before fixation. The label tolerates permeabilization and can be combined with intracellular immunofluorescence.

Biotium CF® Dye Phalloidin

For F-actin and cytoskeleton imaging in fixed cells, including super-resolution microscopy applications.

CST IF-validated antibodies

For investigating protein localization, expression and activation state in fixed cells and tissue specimens. Suitable for multiplex immunofluorescence workflows.

Challenging tissue samples and signal optimization

Vector TrueVIEW® Autofluorescence Quenching Kit

For reducing tissue autofluorescence, including in formaldehyde-fixed and FFPE samples. Enhances signal-to-noise while retaining specific IF staining.

Vector VECTASHIELD® Mounting Media

For protecting fluorescent signals against photobleaching in fixed cell and tissue samples for epifluorescence and confocal imaging.

Vector VectaFluor™ Excel

A non-biotin fluorescence signal amplification system for low-abundance targets in immunofluorescence.

Dynamic imaging of a protein of interest

NEB SNAP-tag® & CLIP-tag™

For selective fluorescent labeling of a protein of interest in live or fixed cells. Supports dual-color protein visualization and pulse-chase experiments.

Imaging Systems

 

Celena X

CELENA X is a fully automated high content imaging system. Equipped with a powerful and flexible software, CELENA X is designed for rapid high content image acquisition and analysis. With the CELENA X you can create and customize workflows that can be used for the simplest fixed cell assays to more complex Z-stack time-lapse live cell assays. The sophisticated imaging software accommodates a wide range of cell-based applications and assays, such as cell counting, wound healing, cytotoxicity, transfection, cell growth and viability, calcium mobilization, organoid imaging, and more.

Features:

  • Laser autofocusing modules and motorized XYZ stage
  • Multicolor fluorescence (4 colours), brightfield, color brightfield, and phase-contrast imaging
  • Interchangeable filter cubes and objectives
  • Compatible with onstage incubation system
  • Video option

Celena S

CELENA S is an all-in-one compact imaging system for manual image capture and analysis. The system is equipped with advanced optics and adjustable transmitted light to produce vivid, high-resolution images of publication quality. User-friendly and powerful data analysis software makes it easy to acquire and analyze multicolor fluorescence, brightfield, Z-stack and time-lapse images, as well as monitor cell growth, cell counts, and viability.

Features:

  • All-in-one system
  • Multicolor fluorescence (3 colours), brightfield and phase-contrast imaging
  • Interchangeable filter cubes and objectives
  • Compatible with onstage incubator syste