Webinaaritallenne BioNordikan virtuaalisesta NGS-tapahtumasta

Järjestimme syksyllä 2022 sekvensointiaiheisen iltapäiväwebinaarin yhdessä toimittajiemme kanssa. Webinaari on nyt katsottavissa tallenteena, ja voit katsoa sen omaan aikatauluusi sopivasti täältä. Seuraava Nordic NGS Meeting järjestetään live-tapahtumana Kööpenhaminassa, 5.-6.10. - laitathan päivät kalenteriin!

Webinaari käsitteli seuraavia applikaatioita:
- CUT&RUN (Cell Signaling Technology)
- 16S microbiome NGS (ViennaLab)
- Näytteen pilkkominen (short- and long-read workflows) (Diagenode)
- NGS-workflow rakentuu modulaarisesti NEBNext-tuotteiden avulla (New England Biolabs)
- Näytteen fraktiointi (Sage Science)
- NGS-kirjaston kvantitointi (EdgeBio)

Löydät haluamasi aiheen tallenteelta ajan perusteella seuraavasti:
00.00 - 30.30: Cell Signaling Technology
31.00 - 1.03.00: ViennaLab
1.03.00- 1.34.30: Diagenode
1.35.00– 2.02: New England Biolabs
2.08.30 - 2.25.30: Sage Science
2.31.30 - 3.00.11: EdgeBio

Alkuperäinen ohjelma ja kuvaus luennoista:

14.00-14.30 Cut&Tag
Speaker: Maxime Jacquet, PhD, Application Specialist, Cell Signaling Technologies

CUT&Tag, cleavage under targets and tagmentation, fragmentation and libraryprep in one step for protein interactions with DNA.

14.30-15.00 16S Microbiome NGS Assay. The highly efficient workflow for microbiome sequencing
Speaker: Anna Malolepszy, PhD, NGS Product Specialist, ViennaLab

Human gut is inhabited by countless amounts of bacteria, viruses and fungi. The composition of our gut is a very dynamic environment, changing throughout our lives and it is affected by the lifestyle, diet, antibiotic intake etc. The microbiome imbalance was implicated in many human diseases, such as obesity, type II diabetes, inflammatory bowel diseases, asthma to name a few. Therefore, it is becoming of more importance to investigate and understand the human microbiome and how it can affect human health.
ViennaLab offers a robust and efficient workflow for the 16S microbiome NGS Assay, which allows species-level classification. The protocol is amplicon-based, focusing specifically on V3-V4 region of the 16S rRNA gene. Our all-in-one solution consists of library preparation, bioinformatic analysis and report generation, which will be described in details in this webinar.

15.00-15.30 High-quality sample shearing in short- and long-read workflows
Speaker: Juri Kazakevych, Customer Support Specialist, Diagenode

With short- and long-read sequencing applications developing at a fast pace, the need for reproducible - and often high-throughput - sample preparation remains constant. In this session we will compare commonly used fragmentation approaches, discussing optimizable parameters to provide narrow library fragment size distributions while maintaining sample integrity. Additionally we will look at standardisation options to remove the human factor for this key step of library preparation, allowing for reproducible results across processing batches and labs.
We will focus on two main workflows:
• short-read DNA/RNA/chromatin shearing for short-read applications in the 100-1000bp range
• long-read sequencing in the 3-100kb range and handling of viscous HMW-DNA

15.30-16.00 How to overcome common challenges and bottlenecks in NGS Library Prep
Speaker: Michael Petridis, PhD, Application Specialist, New England Biolabs

As sequencing technologies improve and applications expand, the need for compatibility with ever-decreasing input amounts and sub-optimal sample quality grows. Scientists have a broad range of different workflows from various suppliers at their disposal, minimizing the ability to define routine processes. As a consequence, scientists must often balance reliability and performance with faster turnaround, higher throughput and automation compatibility.

During this webinar, we will highlight solutions to overcome these bottlenecks in various DNA and RNA sequencing applications, that will enable you to streamline your NGS library prep, while saving time and reducing cost. Find out how the different NEBNext DNA and RNA Library Prep workflows are tied together and pivot around a centralized core workflow and learn how to benefit from the robustness of these core reagents including in automation.

16.00-16.30 Blue Pippin and PippinHT Long Read Applications
Speaker: Christina MacNaughton, Sage Science

The Blue Pippin and PippinHT are able to perform Targeted as well as High Pass sample collection. High Pass collection is a particularly powerful tool for long read sample prep as it eliminates undesirable smaller DNA sizes - resulting in sample primarily composed of the desired HMW sizes. In addition to High Pass, we have a soon to be released collection mode Range +T which allows for narrow collections of HMW DNA.

16.30-17.00 BrightBox Quantitation Assay – a faster and efficient way to quantitate NGS libraries
Speaker: Avesta Ebrahimi, General Manager, EdgeBio
cell immuno instruments custom