Cell Signaling Technology
Cell signaling lies at the heart of numerous biological processes including all the intricate communication that occurs within and between cells. This complex web of molecular interactions - the signaling pathways- regulate critical cellular processes like growth, proliferation, differentiation, metabolism, and apoptosis. Understanding the intricacies of these pathways is key to understanding how normal cells function and how dysfunction in the signaling cascade(s) leads to disease.
Cell Signaling Technology (CST) is a leading manufacturer providing antibody-based tools and innovations for the study of these cell signaling pathways. CST’s offering includes antibodies, assays, companion products and kits for a wide range of techniques, including western blotting, immunohistochemistry, immunofluorescence microscopy, immunoprecipitation, ELISA, flow cytometry, and proteomics.
Cell Signaling Technology is well known for its antibodies that are validated for each application. CST set the standards for their antibody validation (Hallmarks of Antibody Validation) more than 20 years ago. Hallmarks of Antibody Validation arose from the need to have an antibody that can be trusted each time. The hallmarks are based in the work by Uhlen, et. al. (A proposal for validation of antibodies. Nature Methods 2016).
CST is well known for high-quality rabbit monoclonal antibodies (mAb). Rabbit mAbs typically exhibit higher affinity, specificity and stability compared to mouse monoclonals. Rabbit mAbs have improved resistance to denaturation, temperature fluctuations, and proteolytic degradation. Due to their enhanced affinity and specificity, rabbit mAbs are often preferred for immunocytochemistry and immunohistochemistry applications as they provide more robust staining with lower background, resulting in clearer and more reliable results. Using rabbit mAbs also overcome mouse-on-mouse staining without additional steps in protocol.
CST provides more than 70 different secondary antibodies for applications like immunohistochemistry, Western blotting, immunofluorescence, flow cytometry and immunoprecipitation.
CST offers a selection of products required to complete the experimental workflow. These include epitope-tagged and control antibodies, secondary antibodies, detection reagents, experimental controls, standard buffers, and other reagents. These same reagents are used by CST in validation of their the primary antibodies and therefore they work optimally together with the monoclonals in applications like Western blotting, immunohistochemistry, immunoprecipitation, immunofluorescence, flow cytometry, chromatin immunoprecipitation and ChIP-seq, and more.
Sampler kits provide economical means to study one specific signaling cascade. There are more than 300 kits, most of them for Western blotting. The kits include enough antibody (20 µl primary, 100 µl secondary) to run two Westerns. Larger volumes of the antibodies are available separately, if needed for further studies.